Services
Our Services
01 .Sequencing
02. Next Generation sequencing
03. QPCR Services
04. Gene Expression qPCR Services
05.miRNA expression qPCR Services
06. SNP Genotyping qPCR Services
07. Absolute Quantification qPCR Services
08. Proteomics Services
sequencing
Sequencing
Sanger’s & de-novo Method
de novo sequencing is the term used to describe the initial sequence analysis performed to obtain the primary genetic sequence of a particular organism. A detailed genetic analysis of an organism is possible only after de novo sequencing has been performed.
For de novo sequencing using capillary electrophoresis, the target DNA is fragmented and cloned into a viral or plasmid vector. Cloning provides amplification of the target DNA (by bacterial growth) and allows sequencing primers to bind to known sequence in the vector and extend the sequence into the unknown target DNA.
High Throughput Sequencing (HTS)
High throughput sequencing technologies have become essential in studies on genomics, epigenomics, and transcriptomics. While sequencing information has traditionally been elucidated using a low throughput technique called Sanger sequencing, high throughput sequencing (HTS) technologies are capable of sequencing multiple DNA molecules in parallel, enabling hundreds of millions of DNA molecules to be sequenced at a time. This advantage allows HTS to be used to create large data sets, generating more comprehensive insights into the cellular genomic and transcriptomic signatures of various diseases and developmental stages. Within HTS technologies, whole exome sequencing can be used to identify novel variants and other mutations that may underlie many genetic cardiac disorders.
Next Generation sequencing
Next Generation sequencing
Meta genome Sequencing
Metagenomic NGS (mNGS) is simply running all nucleic acids in a sample, which may contain mixed populations of microorganisms, and assigning these to their reference genomes to understand which microbes are present and in what proportions. The ability to sequence and identify nucleic acids from multiple different taxa for metagenomic analysis makes this a powerful new platform that can simultaneously identify genetic material from entirely different kingdoms of organisms.
The possible clinical applications are tremendous, including diagnosis of infectious diseases, outbreak tracking, infection control surveillance, and mutation and pathogen discovery, among many others. mNGS of clinical samples has been applied to various sample types including cerebrospinal fluid, blood, respiratory samples, gastrointestinal fluid, and ocular fluid.
Whole genome Sequencing
Whole genome Sequencing (WGS) is
the analysis of the entire genomic DNA sequence of a cell at a single time,
providing the most comprehensive characterization of the genome. WGS has revolutionized the biosciences and proven to be essential
and invaluable to the identification of gene functions and their involvement in
disease. The feasibility of WGS analysis is under the support of next generation
sequencing (NGS) technologies,
which require substantial computational and
biomedical resources to acquire and analyze large and complex sequence data.
Meanwhile, the rapid progress and innovation of NGS technology has successfully
enabled the generation of large volumes of sequence data and reduced the expense for WGS. Transcriptome
Transcriptome sequencing encompasses a wide variety of applications from simple mRNA profiling to discovery and analysis of the entire transcriptome, including both coding mRNA and non-coding RNA (e.g., miRNA, small RNAs, linc RNAs). These applications, collectively called RNA-Seq, are extremely popular for next generation sequencing platforms as they uncover information that may be missed by array-based platforms, as no prior knowledge of the transcript sequence is needed. RNA-Seq was initially used primarily for discovery applications (rare genes, splice junctions, gene fusions) and with novel or poorly studied organisms for which there weren’t good standard microarrays. However, as the technology is becoming more available and costs are coming down, it is starting to be used for RNA profiling (sample comparison) as well. Also, as it is sequencing based, it is well suited for specialty applications such as RNA editing and allele specific expression. While there are a variety of RNA-Seq applications and protocols, most follow the basic strategy of isolating RNA (such as with poly dT to pull down mRNA), converting it to DNA and then adding adaptor sequences to generate a library suitable for sequencing.
QPCR Services
QPCR Services
Real-time PCR (qPCR) is the most widely used technology for nucleic acid quantification and genotyping. It offers a complete solution for real time PCR services to examine gene, microRNA expression and SNP targets that are present in a wide variety of samples. These include gene expression, miRNA expression, SNP genotyping, and absolute quantification assays from wide variety of samples.
Gene Expression qPCR Services
Gene Expression QPCR Services
- Services available in TaqMan/ SYBR Green/ EvaGreen chemistries.
- Our SYBR Green and EvaGreen assay design and optimization workflow includes testing for the correct product size and specificity via gel electrophoresis and melt curve analysis. We will also test the amplification efficiency of each assay using standard curves.
miRNA expression qPCR Services
miRNA expression qPCR Services
- Services available with TaqMan miRNA Assays and TaqMan Advanced miRNA Assays.
- Both chemistries can discriminate between miRNAs that differ by a single nucleotide.
SNP Genotyping qPCR Services
- SNP genotyping via qPCR is a more simplified workflow compared to direct sequencing.
- We can perform SNP genotyping using predesigned TaqMan SNP assays.
- If your SNP is novel, then we can assist in designing and ordering a Custom TaqMan SNP assay.
- Please submit ~20ng DNA per sample per SNP.
Absolute Quantification qPCR Services
- Services available in TaqMan/ SYBR Green/Eva Green chemistries.
- Send us your Standard sample, e.g. plasmid, PCR amplified product, or in vitro-transcribed RNA.
- We’ll perform the calculations and dilutions necessary to generate the Standard Curve.
- Based on the Standard Curve results, we’ll quantify the copy numbers of your Unknown samples.
Proteomics Services
Proteomics Services
Mass spectrometry (MS)-based proteomics has emerged as a powerful and universal method for the global measurement of proteins and post translations modifications. Unlike traditional western-blot or ELISA method MS-based proteomics tools can identify and quantify the protein mixtures in the same time at large scale. Quantification of small molecules by liquid chromatography coupled to a tandem mass spectrometer (LC-MS/MS) provides critical quantitative analytical support in both the drug development process and medical/agriculture research. We have extensive expertise in the development, validation and use of LC-MS/MS assays, using a triple quadrupole instrument to perform quantitative analysis in a variety of sample matrices. Matrices previously quantitated by staff at DNA Xperts include, but are not limited to, urine, plasma, tissue (brain, tumour, liver spleen), cells, cell media, cerebral spinal fluid, solutions and formulations. A variety of analytes, both small molecules and biologicals, can be quantified by LC-MS/MS.